SEP Chromatography System

The SEP series products are the latest compact and easy-to-use protein chromatography systems. Its unique design utilizes a dual-valve gradient mechanism to provide a versatile and cost effective solution to address applications in the development labs. It is primarily used for laboratory-level process research and method development, catering to the purification of target products such as proteins, antibodies, vaccines, nucleic acids, and diagnostic materials ranging from micrograms to tens of grams.

SEP Chromatography System-1016
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Technical Features

  • Streamlined design with a simple and flexible configuration, making it easy to get started.

  • One key arrangement of the pipeline, more tidy arrangement.

  • Quick start, pause button, more convenient operation.

  • Detachable tray design, leak-proof safer, easier to clean.

  • The UV detector is fully enclosed and anti-interference.

  • Large valve head design, pipeline disassembly is more convenient.

  • pH valves provide multiple flow path options for calibration and storage without disassembly.

  • PURfect P® software has built-in DOE and Scouting functions for easier conditional screening.

Application

  • Monoclonal antibody

  • Recombinat protein

  • Vaccines

  • Biochemical drug

  • Antibiotics

  • Natural products

  • Polysaccharides


  • Technical Details
  • URS
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Model

SEP-025

System pump

plunger pump, bioinert PEEK material, provides stable while precise flow rates

Flow rate range

0.01-25 ml/min

System pressure rate

0-10 MPa (100bar, 1450psi)

Mixer

Magnetic stirring dynamic mixer, standard size: 2ml

Flow rate accuracy

±1.2%

Flow rate precision

RSD<0.5%

Supported elution

Isocratic/ramp/step gradient, in-process modification available

UV detector

Option (1) F2-Default 260 & 280 nm detector, detecting both channels simultaneously;

Option (2) V2-Detects 2 wavelengths at the same time, wavelength range: 200nm-400nm;

Option (3) V4-Detects 4 wavelengths at the same time, wavelength range: 200nm-400nm;

Option (4) V6-Detects 4 wavelengths at the same time, wavelength range: 200nm-600nm;

Option (5) V8-Detects 4 wavelengths at the same time, wavelength range: 200nm-800nm

Wavelength precision/repeatability

±1nm / ±0.5nm

UV noise / drift

0.16 mAu（1s）/ 1 mAu/h

Conductivity sensor

0.001-999.99 mS/cm, precision ±0.1 mS/cm or ±2%

Temperature sensor

0-100℃, precision±1℃

Chromatography Workstation

PURfect P® advanced chromatography workstation for Win10 operating system

Installation toolkit

PEEK / PTFE pipe, installation manual, user manual, tube connector, column clip, back-pressure valve, pre-column filter, 1 ml quantitative loop, common tools, etc

Inlet valve

Four buffer inlets, A!-A2, B1-B2

Automatic sample injection valve

Load/Inject/Waste mode

pH valve (optional)

0 - 14, precision ±0.1 (2-12)

Column-position valve (optional)

Single valve with 5 column positions; Column, Bypass, reverse flush functions

Collection valve (optional)

Eight outlets（1 waste, 7 collection outlets）

Fraction collector (optional)

Small size, flexible collection methods, can place the same or different specifications of the collection bracket side by side, automatically identify the collection rack.

13mm collection rack: 1 x13mm test tube rack, 90 x 5ml test tubes

16mm collection rack: 1 x 16mm test tube rack, 60 x 15ml test tubes

28mm collection rack: 1 x 28mm test tube rack, 21 x 50ml test tubes

96-well plate collection rack: 1×96-well plate collection rack , containing four deep-well 96-well plates

Material

PEEK, Ti, PTFE

System power

220VAC/400W

Instrument weight (gross weight)

50kg

Dimension (W*H*D)

420mm*523mm*578mm


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User’s Manual

    SEP Chromatography System
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