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Isolation of modified protein

Jan 26, 2024

Chromatographic column

NmLOAD 50/600,NW Super 75

Mobile phase

50mM PB+0.1M NaCl （pH 6.8）

Formulation scheme

6.12g NaH2PO4+6.95g NaH2PO4 +11.68g NaCl, adding water to 2L, ultrasonic dissolution filtration

Flow rate

1.5mL/min

Detecting wavelength

254nm/214nm/280nm

Loadin condition

Quantitative cup sampling, 10mg/ml, injection volume 10ml, 100mg each time

Crude product separation diagram


Target components and crude products


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